ENTRAF

An Encyclopedia of well-annotated DNA-binding TRanscription factors in Bacteria and Archaea.

Transcription factor information

General information

ENTRAF ID ENTRAF0460
Gene name ada
Uniprot ID ADA_ECOLI
Organism Escherichia coli (strain K12)
Aminoacid sequence MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRARHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTLEALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFPDSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGYRWGVSRKAQLLRREAENEER
FASTA file Download
Length 354

Functional information

Function Uniprot Involved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Repairs O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme (Cys-321). Also specifically repairs the Sp diastereomer of DNA methylphosphotriester lesions by the same mechanism, although the methyl transfer occurs onto a different cysteine residue (Cys-38). Cannot demethylate the other diastereomer, Rp-methylphosphotriester. This is a suicide reaction: the enzyme is irreversibly inactivated.
Role Activator; Repressor
Protein family AraC/XylS
Structure (PDB ID) 1ADN

References

Pubmed ID 1 2987251
Pubmed ID 2 3887409
Pubmed ID 3 9097040
Pubmed ID 4 9278503
Pubmed ID 5 16738553
Pubmed ID 6 2982792
Pubmed ID 7 3536913
Pubmed ID 8 3009022
Pubmed ID 9 3529081
Pubmed ID 10 2987862
Pubmed ID 11 3162236
Pubmed ID 12 1581309
Pubmed ID 13 8202360
Pubmed ID 14 9298644
Pubmed ID 15 8500619
Pubmed ID 16 8395079
Pubmed ID 17 8156986
Pubmed ID 18 11284682
Pubmed ID 19 16209950
Pubmed ID 20 16452614

Experimental evidences

BPP, SM, GEA