ENTRAF

An Encyclopedia of well-annotated DNA-binding TRanscription factors in Bacteria and Archaea.

Transcription factor information

General information

ENTRAF ID ENTRAF0479
Gene name birA
Uniprot ID BIRA_ECOLI
Organism Escherichia coli (strain K12)
Aminoacid sequence MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK
FASTA file Download
Length 321

Functional information

Function Uniprot Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
Role Repressor
Protein family BirA
Structure (PDB ID) 1BIA

References

Pubmed ID 1 3899863
Pubmed ID 2 3536662
Pubmed ID 3 8265357
Pubmed ID 4 9278503
Pubmed ID 5 16738553
Pubmed ID 6 1311302
Pubmed ID 7 1328157
Pubmed ID 8 1409631
Pubmed ID 9 11353844

Experimental evidences

BPP, SM